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From 2012.igem.org

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Contents 1 Wetlab Notebook 2 Drylab Notebook 3 Outreach Notebook 4 Strain List 5 Old Links 5.1 Subprojects 5.2 Protocols

Wetlab Notebook

WETLAB NOTEBOOK. PUT EVERYTHING HERE.

Drylab Notebook

DRYLAB NOTEBOOK.

Outreach Notebook

OUTREACH NOTEBOOK.

Strain List

Strain List

Old Links

Subprojects

DO NOT DO ANY EDITING ON THE BELOW LINKS. THE BETTER VERSIONS ARE THE ABOVE. THESE ARE ONLY STILL HERE FOR REFERENCE AS A "JUST IN CASE"

• Arsenic reporter -- test a system for Shewanella detecting arsenates and activating the Mtr pathway to produce an electrical current
• nah operon -- an operon that converts napthalene into salicylate, allowing it to be detected by the salicylate reporter.
• Salicylate reporter -- a system for Shewanella detecting salicylate and activating the Mtr pathway to produce an electrical current

Protocols

• Miniprep
• Vent PCR
• Phusion PCR
• Colony PCR
• Gel Electrophoresis
• Gel Purification
• DNA Quantification
• Digestion Reaction
• Dephosphorylation
• PCR Clean Up
• Ligation Reaction
• Transformation of E. coli
• Transformation of Shewanella
• Sequencing
• Gibson Assembly
• Making LB
• Autoclave
• Making Super Optimal Broths