Team:UC Chile/Cyano/Notepad/week7

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Cyanolux & SpiderColi - Pontificia Universidad Católica de Chile, iGEM 2012



notebook


Week 7

Tuesday 04.17

We purified parts for Construct 1 and then assembled them by Gibson. Hope everythings works out. Simon was wearing his Gibson Les Paul T-shirt so we kinda hope that the power of rock leads us through the good side of the force.

Also, have you ever seen a centrifuge dance? It's pretty funny actually.In one step of the purification, the protocol stated "centrifuge with lid open"

So we did...and it danced...

Pic of the day

Proudresize.jpg



Thursday 04.19

Construct 1 is ready!!!

Freddie.jpg


Conclusion: absolutely nothing is more powerful than rock!


Friday 04.20


Seven a.m., waking up in the morning. Gotta be fresh, gotta go downstairs...

hahaha i'm just messing with your head


Sat 04.21

Started preparing parts for Construct 2 by PCR.

Here is Simon heroically diminishing his life span for the success of Construct2.

Lifespan.jpg

Although he actually seems to be enjoying it...

Enjoys.jpg


To do:

Assemble C2.1 and C2.2. Characterization of psAB and psbA2 promoters.