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Team:Cambridge/Week 3/Lab book
From 2012.igem.org
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Contents |
Monday
Making bacillus competent
- Bacillus salts *10 made up and autoclaved.
- Medium A base *10 made up (glucose will be added tomorrow)
- bacillus strain 168 streaked out and grown on plate overnight.
Tuesday
Making bacillus competent
- Filtered glucose added to sterile Medium A base.
- Sterile aliquots of salts and medium apportioned and put in fridge (10*50ml each)
Wednesday
Making bacillus competent
Thursday
Test transformation of frozen bacillus stocks
- Two samples of bacillus defrosted from different batches.
Friday
Transformation of bacillus and e.coli with lux genes from 2010
Testing of the spectral properties of filters
- Blue , Green and Red filters were tested in different wavelengths (400 nm - 600 nm) in a spectrophotometer.
- Excellent absorbance/emmitance results given by the blue and red filters near 580nm and 490nm respectively. 580nm is the wavelength where intensity of light is to be measured to identify the presence of mOrange. 490nm is the wavelength where maximum light intensity is expected with or without the presence of mOrange.
