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From 2012.igem.org

• PCR using Phusion DNA polymerase A method for amplifying a section of DNA.
• Colony PCR PCR with cells as a template. Useful for checking the length of an insert in an introduced plasmid.
• Gibson Assembly A technique for ligating multiple DNA fragments in one step, compatible with standard assembly.

N.B. Information in purple is subject to change through optimisation over the course of our project.

Notebook