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From 2012.igem.org

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Contents 1 July 8th-14th 1.1 July 9th 1.2 July 10th

The salicylate reporter is analogous to the arsenic reporter, but with a salicylate-sensitive promoter. That is, in the presence of salicylate it will produce MtrB, completing the Mtr pathway and allowing Shewanella to produce current in our biosensor. In combination with the nah operon, this reporter will be able to detect naphthalene levels. Back to salicylate reporter week view

July 8th-14th

July 9th

Dylan performed a Phusion PCR with a single annealing temperature of 59 degrees Celsius using primers 27 and 28 for the final time. In the future, new primers will be used. The purpose of this PCR was to amplify p21. The PCR product was analyzed using agarose gel electrophoresis to determine if the desired 1.127 kb fragment is present. To transform our plasmids into Shewanella with conjugation, we had asked Dr. Jeff Gralnick for WM3064 E. coli for which plates containing 2,6-Diaminopimelic Acid (DAP) are required. In preparation to receive this strain, the plates with DAP were prepared.

July 10th

Caleb miniprepped strains P8, P9, P10, P15, and P16 (See our strain list) Also, our new freezer arrived. We cleaned and welcomed our new friend. The WM3064 came in and we began to grow greater volumes of the cells. Dylan set up two 18 mL/hr continuous flow reactors using 10x diluted LB and innoculated one the reactors with JG700 and one with wild type Shewanella oneidensis MR-1

==July 11th==