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Team:Nevada/Protocols
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Phusion PCR
Thermocycling conditions:
Initial Denaturation: 98°C for 30 seconds
25-35 cycles:
- 98°C for 10 seconds
- 55°C, 60°C, 65°C for 15 seconds
- 72°C for 15 seconds
Final Extension: 72°C for 5 minutes
Qiagen Miniprep kit
www.qiagen.com/hb/qiaprepminiprep
