Team:Cambridge/Protocols/M9Medium

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This is a detailed record of our work during the Summer...Read More

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M9 Minimal Medium

M9 salts x5

64 g Na₂HPO₄.7H₂O
15 g KH₂PO₄
2.5 g NaCl
5 g NH₄Cl

dissolved in deionized water to a final volume of 1 L

Autoclave before use.

Final medium

1x M9 salts
2mM MgSO₄
0.1mM CaCl₂
0.4% carbon source (e.g. glycerol, glucose, etc.) - filter sterilized.
In sterile H₂O

NB - Do not autoclave final medium - carbon source will caramelize.

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