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Team:Trieste/notebooklast
From 2012.igem.org
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The Jolly JoCare
a safe probiotic platform for protein expression
Last month
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Suicide System
We tried to complete the construct, cloning the LL 37 construct inside the Holin ones, but unfortunately we didn't obtain positive colonies.Antibody
The last month we tested the our antibodies. First, we tried to prove that the antibodies fused with LPP-OmpA fragment are actually localized into the outer membrane. To this end, we performed the FACS analisis, but we soon abandoned this approach because it was non suitable for our purpose. Then we performed an immunofloresence assay on the recombinant bacteria. This technique was more successfull. In order to test the functionality of Ab, we did ELISA test.Cumate-Switch Regulation
CymRWe tried several times to clone the single J23100-CymR-B0015 Eco/Pst into the plasmid K510012 but we didn't have positive colonies.
