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From 2012.igem.org

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From Biofilms to Biofuel Project Flowchart Caltech’s 2012 iGEM team aims to manipulate bacteria to degrade stable organic polymers such as lignin and alginate; to use these substrates to synthesize biofuels, specifically biodiesel; and to direct their ATP synthesis mechanism to rely on proteorhodopsin as the source of the proton gradient, freeing NADH to interact in the synthetic pathway instead. We will develop the three facets of the project in different strains of E. coli. We will then splice the manipulated sequences into one new organism.

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