Team:Peking/HumanPractice/Outreach/Collaboration

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Summary

This summer, our team benefitted a lot from the collaboration with Prof. YANG Yi and other iGEM teams. By paying reciprocal visits with Prof. Yang, we establish collaboration with his lab. By helping some other teams with their contests, we built lifelong friendship with team members across the world.

Collaboration with Prof. YANG Yi's Lab

As a burgeoning interface of biology, chemistry, physics and many other disciplines of research, the study of synthetic biology highly values and is critically depended on the collaboration of different specialities and different groups. Aiming at the optimization and further application of an ultra-sensitive light sensor, our team decided to collaborate with Prof. YANG Yi’s group from ECUST (East China University of Science and Technology) and research under the instruction of Prof. YANG.

Upon his visit to Peking University this May, Prof. YANG presented his design and preliminary results on the Light-OFF system and we shared our opinions on its optimization and further applications. And in this very meeting, both sides decided to combine each component of their works and present this project in the iGEM competition.

Prof.Yang Yi

Professor YANG Yi

Based on the original work of YANG’s group, team members in Peking University endeavored in the possible extension of this light sensitive and functional Light-OFF biobrick.

Collaboration with Other iGEM Teams

SJTU_iGEM

SJTU_iGEM

Another important part of iGEM is helping other teams through communication and collaboration. Team SJTU-BioX-Shanghai found that we utilized ribozymes last year to control the expression of VioABDE gene, and thereby requested biobricks of BBa_K598019 and BBa_K598020 to incorporate in their "Membrane Magic" project. We shared the DNA materials with them.

BBa_K598019

BBa_K598019

TJU_iGEM

TJU_iGEM Team

We also collaborated with Team Tianjin by sharing ideas, protocols and information. Later, we helped them to characterize their biobricks BBa_K821001 and BBa_K821002 using fluorescence stereomicroscope.

OUC_iGEM

OUC_iGEM Team

Also we established a positive, cooperative, and steady relation with OUC-China. Our team and OUC iGEM helped each other a lot this summer. We helped them to build stochastic model and gave some important advice about their program, which provides guidance for the experimental implementation. Reciprocally, they shared DNA material of agarase, an enzyme that degrades agar, with us, which is very useful for our 3D bio-printing.

OUC_iGEM Result

SYSU_iGEM

SYSU_iGEM Team

Besides, we also shared DNA materials of BBa_K598002 with Team SYSU-China. They exploited this digital-like and plug-in toggle switch to implement sequential logic processing in their gene circuit.

Bistable

Fluorescence images of bistable switch.

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