Team:Potsdam Bioware/Lab/Labjournal/October

AID

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Inoculation of plasmid samples of the 48h retransformation plates (after FACS)

Investigators: Tom

Time: 2012-10-10

Materials:

• LB medium
  

• Amp stock solution
  

• plate with cultures:
  

EGFR-C-AID without NES, with NLS+Kozak sequence+eGFP

(after FACS selection)

Method:

Inoculation of:

5 cultures per plate in 5 ml LB medium + 5µL Amp.

(--> 5 cultures)

Further tasks:

• Miniprep
  

Antibody

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Virus

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Further tasks