"
Team:Lethbridge/Acetic Acid Production
From 2012.igem.org
Results
Acetic Acid Production
Acetic Acid Production and Export by E. coli
Overview
To test the effectiveness of overexpressing and co-localizing PTA and ACK with the transporter Aata, we first needed to determine intrinsic acid production by Escherichia coli BL21(DE3). Glucose was used as an energy source for E. coli, since this will be produced by Synechococcus elongatus in our optimized system.
Experimental Setup
Overnight cultures of E. coli were used to inoculate fresh LB media that was supplemented with various concentrations of glucose (0-16 g/L). Growth of the cultures was monitored by measuring the optical density at 600 nm every hour for 6 hours. In addition, 4 mL of each culture were collected and centrifuged to obtain a clear supernatant. Titrations were performed with standardized 0.05 M NaOH in order to determine the concentration of acid in the media.
