Team:Queens Canada/Guide/Protein Structure

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Control

Guide to making Chimeric Proteins- Protein Expression

Linkers
Structures on PDB
Issues
Expression

Linkers

In our chimeras we have linker sequences on either side of the chimeric insert sequence. The linkers we chose to use were inert, and flexible linker sequences, with different sequences on either side so that overlap extension will be successful.

FliC -GGGGS- insert -GGSGG- FliC

Structures

Reading_frame

Rare_Codons

CohDoc