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Contents 1 Hello 2 March 2.1 Spring Boot Camp 2.1.1 Monday, March 5 2.1.2 Tuesday, March 6 2.1.3 Wednesday, March 7 2.1.4 Thursday, March 8 2.1.5 Friday, March 9 3 July 3.1 4th 3.2 5th 3.3 6th

Hello

We are team HokkaidoU Japan! Today we learn and start to edit wiki. (>ω<)　
Dear Mr.Ortiz, I saw the help page which you edited.
hola!

March

Spring Boot Camp

date

March 5 (Mon) ~ March 9 (Fri)

Monday, March 5

Session #1

Short lecture about moleculer biology (Mr.Yamazaki, our adviser)

Session #2

Tutrial: How to use 'Unipro UGENE' (iTakeshi)

Session #3

Guidance: Wiki Reading (Laury)

Example: 2010 MIT

Tuesday, March 6

Session #4~6

Reading Wikis in turn and discussions

• 2010 NYU
• 2009 Cambridge
• 2009 Growningen

Wednesday, March 7

Session #7~11

Reading Wikis (2)

• 2010 Washington
• 2009 Valencia
• 2011 Barklay
• 2010 Paris
• 2010 Bristol

Thursday, March 8

Session #12

2012 Project Brainstorming

The details is secret! :)

Session #13

Guidance: How to read papers (Laury)

Friday, March 9

Session #14

2012 Project Brainstorming (2)

Session #15

Guidance: How to look up papers you want (Laury)

Session #16

Tutorial: Modeling the behavior of cells (iTakeshi)

Session #17

Final Session: Reviewing this camp

Party!!

July

4th

Transformation

• Transformation of BBa_B0015(dT), B0034(RBS), I179005(pT7), K346007(Ag43), and K542009(pLacI-RBS-Ag43)in DH5α
  • Cultivated on LBA(dt,RBS,T7) and LBC(Ag43, pLacI-RBS-Ag43)
    • T7:21hrs Others:20hrs

5th

Transformation

• Transformation of K346007(Ag43)in DH5α.
  • Cultivated on LBC in 21hrs.

Single colony isolation

• Single colony isolation of BBa_B0015, B0034, I179005 and K542009.
  • Cultivation on LBA(dt,RBS,T7) and LBC(pLacI-RBS-Ag43) in 14hrs30mins
    • BBa_K542009 was Ag43 only part! And the part didn't have Biobrick suffix.

6th

Liquid culture

• Liquid culture in LBA(dT,RBS,pT7) and LBC(pLacI-RBS-Ag43)
  • LB : 1ml is for glycerol stocks and 2ml is for mini-prep.

Single colony isolation

• Single colony isolation of K346007(Ag43).