User:DrJones1935/7 June 2012

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Laboratory Notebook for Spencer Katz

Lab Notes, Thurs, 7 Jun 2012

I. Inoculate Wild Type (WT) B. subtilis culture from Altman Lab in Nutrient Broth (Lim)

  • Add ~12.5 mL of Nutrient broth to sterile 125 mL E-flask, re-cover with tin foil
  • Inoculate 1 colony from pre-existing plate culture with inoculating loop to flask (11:05 AM)
  • Grow at 37 oC with shaking overnight
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