Team:Berkeley/Project/Zippers

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iGEM Berkeley iGEMBerkeley iGEMBerkeley

Mercury

Protein interaction domains (coiled coils, PDZ, SH3, GBD, etc) have been used by synthetic biologists to oligomerize or localize proteins. In particular, protein-protein interaction pairs have been applied to both scaffolding and signaling applications with impressive results. In such systems, orthogonality of protein pair interactions is desired to reduce cross talk between system components and allow synthetic biologists to gain greater control over a system. However, the limited number and diversity of well-characterized, orthogonal protein interaction domains restricts the complexity of systems that can be designed. Current screening methods for protein-protein interactions (such as transcription-driven GFP expression in yeast 2-hybrid systems) do not have high enough throughput to analyze large library sizes of protein interaction pairs. However, with the utilization of MiCodes and microscopy’s ability to record spatial information, we are able to design an assay to screen for orthogonal protein-protein interactions.


Why we bound mKate to bait and PTS1 to prey. Includes images showing preliminary results.


Our home run experiment with 40 bait and 40 prey.