"

From 2012.igem.org

• Home
• Team
  • Mentors
  • Undergraduates
  • CalArts
• Project
  • Overall Project
  • Materials & Methods
  • Degradation
  • Proteorhodopsin
  • Bacterial Animation
• Parts
  • Submitted Parts
  • Characterization
• Results
  • Degradation
  • Proteorhodopsin
  • Bacterial Animation
• Notebook
  • Degradation
  • Proteorhodopsin
  • Bacterial Animation
  • Materials & Methods
  • Safety
• Sponsors
• Human Practice

Proteorhodopsin Notebook

6/22/12

Julia - transformed K572005 (proteorhodopsin gene)
Daisy - ordered primers for Polymerase Cycling Assembly (PCA) reaction to create a proteorhodopsin optimized for E. coli
Back to Proteorhodopsin Notebook Calendar
6/25/12

Chenxi - Transformed pKD46 into wild type MG1655 E.coli strain using electroporation; designed primers to PCR pKD4 Kan resistance gene for lambda red knockout of nuo and ndh genes (NADH dehydrogenases)
Julia - sequenced K572005
Back to Proteorhodopsin Notebook Calendar
6/26/12

Chenxi - PCR pKD4 kan for nuo and ndh; ran gel
Back to Proteorhodopsin Notebook Calendar
6/27/12

Back to Proteorhodopsin Notebook Calendar
6/28/12

Back to Proteorhodopsin Notebook Calendar
6/29/12

Back to Proteorhodopsin Notebook Calendar
6/30/12

Back to Proteorhodopsin Notebook Calendar
7/1/12

Back to Proteorhodopsin Notebook Calendar
7/2/12

Back to Proteorhodopsin Notebook Calendar
7/3/12

Back to Proteorhodopsin Notebook Calendar