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From 2012.igem.org

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  • Overall Project
  • Materials & Methods
  • Degradation
  • Proteorhodopsin
  • Bacterial Animation
• Parts
  • Submitted Parts
  • Characterization
• Results
  • Degradation
  • Proteorhodopsin
  • Bacterial Animation
• Notebook
  • Degradation
  • Proteorhodopsin
  • Bacterial Animation
  • Materials & Methods
  • Safety
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• Human Practice

Results Page

Degradation

First, vectors that can stably replicate in Z. mobilis were constructed. Using the techniques Gibson assembly and homologous recombination in yeast, new vectors called pMQ95+tet and pMQ97+tet were constructed from replacing the original gene resistances on pMQ95 and pMQ97 with tetracycline and transformed into E. coli colonies. The next step was to identify and obtain the genes that code for the degradation enzymes of allose. It was found that one of the standard laboratory strains of E. coli had the genes for allose degradation enzymes, which are collectively called alsBACEK. This collection of genes was amplified out of the E. coli genome and used to construct new expression plasmids by joining the vectors and alsBACEK. Finally, the plasmid was transferred into Z. mobilis using 4 parent conjugation.

Bacterial Animation

For the results of the bacterial animation project, we have successfully created two constructs that we have submitted to the Parts Registry as BioBricks: mCherry-LVA in pSB1C3 and mCherry-AAV in pSB1C3. We ran a RFP assay on our two parts and found that _______. We are still in the progress of making the bacterial animation system. On the side, we also had an exhibit at Cal Arts on bacterial plate art and had students draw on and make their own plate art.