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From 2012.igem.org

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  • Materials & Methods
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• Human Practice

Materials and Methods

Here is a list of all the procedures we used this summer. Click on a protocol to read its description and the materials required.

General Protocols

• Electrocompetent Cells
• Transformation by Electroporation

Transformation from Distribution

Cell Transformation

Gene Assembly

Overlay Plates

Enrichment Cultures

Rich Media

LB Plates

Colony PCR

Electrophoresis

Yeast Transformation

Conjugation

Degradation Protocols

• Feed the fish
• Check email
• Clean the floor
• Wash the clothes

Proteorhodopsin Protocols

• Polymerase Cycling Assembly (PCA)
• Circular Polymerase Extension Cloning (CPEC)
• Clean the floor
• Wash the clothes

Biofuel Protocols

• Ethanol Assay
• Check email
• Clean the floor
• Wash the clothes

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