E.Z.N.A.™ High Performance Plasmid Mini Kit I (http://www.omegabiotek.com/files/resource/Handbook/09324200.pdf) E.Z.N.A.® Gel Extraction Kit （http://www.omegabiotek.com/files/resource/Handbook/44320600.pdf） TIANprep Mini Plasmid Kit (http://www.tiangen.com/asset/imsupload/up0168548001342417710.pdf) TIANgel Midi Purification Kit （http://www.tiangen.com/asset/imsupload/up0677301001342419001.pdf） DNA Double Digestion Protocol (https://static.igem.org/mediawiki/2012/8/84/DNA_double_digestion_protocol.pdf) Immunofluorescence Protocol (https://static.igem.org/mediawiki/2012/2/25/Immunofluorescence_protocol.pdf) PCR Protocol (https://static.igem.org/mediawiki/2012/3/37/PCR_protocol.pdf) Pichia Yeast Transformation Protocol (https://static.igem.org/mediawiki/2012/0/02/Pichia_Yeast_Transformation_protocol.pdf) Ligation of Insert DNA into Plasmid Vector DNA Protocol (https://static.igem.org/mediawiki/2012/4/46/Protocol_for_ligation_of_insert_DNA_into_plasmid_vector_DNA.pdf) Yeast DNA Rapid Isolation Protocol (https://static.igem.org/mediawiki/2012/1/1d/Protocol_for_Rapid_Isolation_of_Yeast_DNA.pdf) Competent Cells Preparation Protocol (https://static.igem.org/mediawiki/2012/3/3d/Protocol_for_preparation_of_competent_cells.pdf) SDS-PAGE Protocol (https://static.igem.org/mediawiki/2012/9/96/SDS-PAGE_Protocol.pdf) Transformation Protocol (https://static.igem.org/mediawiki/2012/7/75/Transformation_protocol.pdf)