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Team:Korea U Seoul/Notebook/Jun
From 2012.igem.org
Notebook : June
Today, we discussed about main topic and preliminary topic which are bacterial logic gate and carbon fixation using carbonic anhydras
Jeongmin Lee, Kyeongjin Kim: DNA logic gate
reviewed a paper on DNA gate by KAIST 2012
Possible to make logic gate (XOR, NOR, AND, OR) based on DNA
Mechanism: DNA hairpin: input DNA is complementary to output DNA which releases florescence.
Half adder possible, but requires further research on full adder
Hyunkee KimDeoxyribozyme based logic gate
Jang Kyeongwoo JangProgress on XOR logic gate
Possible candidate for XOR logic gate is .Lux R operon and Las R operon which is orthologous to each other
Promoter design
① las - lux - output
② lux - las – output
output is the same protein. When two signals are simultaneously given, each operon inhibits transcription. In effect, this results in no transcription at all
Jihoon Jungresearched on output-input chemicals
One was substance that uses quorum sensing
For example, AHL(Acyl-homoserine lectone)
Since each bacteria uses different AHL, signal won’t mix up
Sanghoon Hanresearched on output-input chemicals
2) Next meeting
Carbonic anhydrase : Byeongnam Min, Haerim Song, Ji Hyoung Nam
DNA logic gate : Jeongmin Lee, Kyeongjin Kim, Wonuk Lee
Plasmid logic gate : Hyunkee Kim, Jang Kyeongwoo Jang, Jihoon Jung, Sanghoon Han
Half adder, full adder completion: everyone
Today, we discussed about main topic and preliminary topic which are bacterial logic gate, Molecular Beacon, and carbon fixation using carbonic anhydrase.
Jeongmin Lee, Kyeongjin Kim: DNA logic gate
Possible to make logic gate (XOR, NOR, AND, OR) based on DNA
Nearly impossible if more than half adder is used. (Note: longer DNA is required).
As DNA gets longer, price for synthesis is getting higher and even more when protein is attached to DNA.
Hyunkee Kim, Kyeongwoo Jang, Sanghoon Han, Jihoon Jungneed to unify input signal and output signal
Improvement on modeling of full gate.
If input and output are the same, these can interfere in signal transfer.
That is to say, input chemical must be destroyed once it gives signal to adjacent promoter..
Back up idea team ( Jihyong Nam, Byeongnam Min, Haerim Song, Dohoon Kwon)Haerim Song: Inhibition of virulence of bacteria on plant – type 3 secretion
Sponsor (Dohoon Kwon, Wonuk Lee)in order to get funding, we need to have presentation on our research or submit a report.
2) Next meeting
From next week, we will have our regular meeting on Wednesday 8p.m
Before next meeting, research the problems and limitations that we discussed during our regular meeting
