Team:Peking/Project/Communication

Introduction

As a significant component in signal transduction, cell-cell communication has fueled numerous biological researches; among them is the discovery of quorum sensing signals, e.g. AHL and AIP. During the last decade, many insightful and valuable synthetic biology projects have been constructed to perform complex functions based on cell-cell communication, e.g. pattern formation and synthetic ecosystems.

However, it is difficult for these systems to perform long-distance signaling, such as in the case of synchronizing cells in a large population, due to the short-range diffusion of chemicals. But an even more serious issue is the basis of synthetic systems on quorum sensing signals, which are difficult to reset because the chemicals are easily saturated in many cases. Additionally, it is difficult to achieve inter-kingdom communication through quorum sensing signals due to the fact that the transcription machinery of prokaryotes and eukaryotes are dramatically different.

As demonstrated above (data on Characterization), the ultrasensitive Luminesensor is able to respond to very dim light and maintains a wide dynamic range. All of this encouraged the Peking iGEM team to explore the possibility of cell-cell communication through light. The delivery of light signals is not limited by diffusion or by a variety in organisms across species or even kingdoms. By carefully selecting the lux operon (bacterial luciferase) as the light sender module (details on Design), Peking iGEM has successfully demonstrated that the Luminesensor is able to sense the blue light produced by bacterial luciferase. This is the very first time that light-communication between cells has been achieved without direct physical contact. As a proof of concept, a video was recorded to reveal the course of change of both the sender and the receiver cells. Quantitative data was also obtained to evaluate the efficiency of light-communication (see Results). To build a complete light-communication system, a Light-On system was also proposed to achieve both positive and negative control by light. As the application of synthetic biology is coming of age, Peking iGEM has probed into the bright future of light-communication (see Prospective).

Figure 1. The treatment to each group(upper) and the result(lower). 1 and 2 contain cells grown in the communication set-up, but for group 2 there is no light emitting cell in the conical flask but only wild-type DH5α. 3 and 4 contain the same light sensing cells grown in test tubes under dim LED (3) or in pure darkness (4).