Team:EPF-Lausanne/Protocol/Gel

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Protocol: Gel electrophoresis


Agarose concentration depends on the size of the DNA to be run. Example using 1% agarose gel and small gel box (80 ml of gel):

  • Add 0.8 g of agarose to a 80 ml clean glass bottle.
  • Pour 1.6 ml of 50xTAE in a graduated cylinder. Fill up to 80 ml with di water.
  • Add the resulting 80 ml of 1xTAE to the glass bottle with agarose.
  • Microwave, at 7, the bottle (no cap!) until it boils.
  • Carefully remove bottle (can be super heated!) and check for the total absence of particles. Microwave again if needed.
  • Prepare a small gel box and fill it up with the agarose solution (maybe not the whole solutio is needed).
  • Add 3 µl (0.05 µl per ml of gel in the box) of Red Gel (it's in the iGEM drawer) and stirr until disolved.
  • UNFINISHED