Team:Paris-Saclay/Project/Notebook/Week 4

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  • We place an order for the primers
  • DNA (biobricks) Resuspension using iGEM protocol
  • Transformation of each biobricks by thermal shock to have a large quantity of DNA
  • Staggering of the transformed bacteria on a selective medium to select bacteria which received the plasmid
  • We put some colonies, for each biobricks, on liquid culture to be able to make a Miniprep
  • Miniprep to purify each biobrick (ready to use)
  • Groove for two colonies because they did not grown on the liquid culture


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