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Promoters & Reporters workgroup

DNA Concentrations
unit= ng/microliter
CFP : 240,1
GFP : 209,1
RFP : 142,8
YFP : 196,0
pCS2+ : 362,7

MiniPrep digestion
CFP, GFP, RFP, YFP : EcoRI/SpeI(BcuI)
Supermix: 10microL buffer Red + 1microL BSA + 74microL
Mix for each sample : 21,25microL supermix + 1microL DNA + 0,5microL EcoRI + 0,5microL BcuI
pCS2+ : EcoRI/XbaI
2,5microL buffer Orange + 0,25microL BSA + 18,5microL ddH2O
=> 3h, 37°C