Notebook

Our team pushed forward an experiment while making trial and error every day.

Creating parts of Methylation and Azurin

Creating parts of Tar

Date:9/13 PCR and Colony PCR

Date:9/14 Electrophoretic confirmation

Date:9/15 Restriction enzyme processing

Date:9/16 Electrophoresis of the restriction enzyme processing product and Restriction enzyme processing

Date:9/17 Refinement of DNA and Ligation,Transformation

Date:9/18 Making of a master plate and Preculture,Recultivation

Date:9/19 Colony PCR and Electrophoresis

Date:9/20 Ligation and Transformation

Date:9/21 Inoculation

Date:9/22 Making of a master plate and Colony PCR,Electrophoresis,Preculture

Date:9/23 Electrophoretic confirmation and Electrophoresis of the PCR

Date:9/24 Miniprep and Electrophoresis

Date:9/25 PCR of the miniprep product and Electrophoresis

Creating parts of EnvZ

Date:9/10 Design of the primer and PCR,Elecrtophoresis,Miniprep

Date:9/13 PCR and Elecrtophoresis

Date:9/18 Design of the primer

Date:9/19 PCR and Elecrtophoresis

Date:9/20 Restriction enzyme processing

Date:9/21 Ligation and PCR,Elecrtophoresis

Date:9/23 PCR

Date:9/24 The refinement of DNA and Restriction enzyme processing,Ligation

Date:9/25 Transformation

Date:9/26 Liquid culture