Team:TU-Delft/Seq NR1
From 2012.igem.org
Gpr109A
EDM13619.1| G protein-coupled receptor 109A [Rattus norvegicus]
Prefix-Promoter(GPD)-BamHI-Kozak-Start-flagtag-gene-Stop-Nde1-Terminator(CYC1)-Suffix
Gaattc gcggccgcttctagagCGGTAGGTATTGA
TTGTAATTCTGTAAATCTATTTCTTAAACTTCTTAAATTCTACTTTTATAGTTAGTCTTT
TTTTTAGTTTTAAAACACCAGAACTTAGTTTCGACGGATGGATCCaAaAaA
ATGGACTACAAGGACGACGACGACAAGGAGCGAAGGAACCACAGTGGGAGAGTGAGTGA
ATTTGTGTTGCTGGGTTTCCCAGCTCCTGCCCCACTGCGAGTACTACTATTTTTCCTTTC
TCTTCTGGCCTATGTGTTGGTGTTGACTGAAAACATGCTCATCATTATAGCAATTAGGAA
CCACCCAACCCTCCACAAACCCATGTATCATCTCAAATCCTGGAAATCCAGCCGGATTTT
CTTGTTCAACCTGGCCGTGGCTGACTTTCTCCTGATCATTTGCTTGCCGTTCTTGACGGA
CAACTATGTCCAGAACTGGGACTGGAGGTTCGGGAGCATCCCCTGCCGCGTGATGCTCTT
CATGTTGGCCATGAACCGACAGGGCAGCATCATCTTCCTCACGGTGGTGGCTGTGGACAG
GTACTTCAGGGTGGTCCACCCGCACCACTTCCTGAACAAGATCTCCAACCGGACGGCGGC
CATCATCTCTTGCTTCCTGTGGGGCATCACCATCGGCCTGACAGTCCACCTCCTCTACAC
GGACATGATGACCCGAAACGGCGATGCAAACCTGTGCAGCAGTTTTAGCATCTGCTACAC
TTTCAGGTGGCACGATGCAATGTTCCTCTTGGAGTTCTTCCTGCCCCTGGGCATCATCCT
GTTCTGCTCTGGCAGGATCATTTGGAGCCTAAGGCAGAGACAGATGGACAGGCACGTCAA
GATCAAGAGGGCCATCAACTTCATCATGGTGGTTGCCATTGTGTTTGTCATCTGCTTCCT
GCCCAGTGTGGCCGTGAGGACCCGCATCTTCTGGCTCCTCTACAAACACAACGTGAGGAA
CTGTGACATCTACTCCTCTGTGGACTTGGCCTTCTTCACCACCCTTAGCTTTACCTACAT
GAACAGCATGCTCGACCCGGTGGTCTACTACTGCTTGCGCAACCAAGATGTCAAAAGAGC
GCTACGTCGCACGCTGCACCTGGCCCAGGACCAGGAGGCCAATACCAACAAAGGCAGCAA
AATTGGTTAATAACATATGTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCC
CCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATT
TATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTT
TTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTT
TTGGGACGCTCGAAGGCTTTAATTTGCGGCCtactagtagcggccgctgcag
Total
amount of nt’s: 1457
Translation
ERRNHS GRVSEFVLLG FPAPAPLRVL LFFLSLLAYV
LVLTENMLII IAIRNHPTLH KPMYHLKSWK SSRIFLFNLA VADFLLIICL PFLTDNYVQN WDWRFGSIPC RVMLFMLAMN
RQGSIIFLTV VAVDRYFRVV HPHHFLNKIS NRTAAIISCF LWGITIGLTV HLLYTDMMTR NGDANLCSSF SICYTFRWHD
AMFLLEFFLP LGIILFCSGR IIWSLRQRQM DRHVKIKRAI NFIMVVAIVF VICFLPSVAV RTRIFWLLYK HNVRNCDIYS
SVDLAFFTTL SFTYMNSMLD PVVYYCLRNQ DVKRALRRTL HLAQDQEANT NKGSKIG