Team:KAIST Korea/Namchini

From 2012.igem.org

‘’’<Data for Our Favorite New Parts>’’’
1. [http://partsregistry.org/Part:BBa_K907000 Main Page] – ‘’’Bacteriophage Bxb1 gp35, DNA integrase(Bxb1_Int), BBa_K907000’’’: A serine integrase family protein which recognizes specific DNA sequences and recombines dsDNA.
2. [http://partsregistry.org/Part:BBa_K907002 Main Page] – ‘’’Binary Signal Generator, RBS(reverse) - attB - Promoter - attP – RBS, BBa_K907002’’’: Promotes transcription and translation of two different proteins one at a time when work with BBa_K907000 and BBa_K907001 to generate binary signal.
3. [http://partsregistry.org/Part:BBa_K907004 Main Page] – ‘’’Dual Phase Protein Generator (GFP default), BBa_K907004: Derivative of BBa_K907002’’’. mRFP and GFP are attached to both ends of BBa_K907002 to generate binary signal via fluorescent proteins.

‘’’<Data for Pre-existing Parts>’’’
1. Experience – ‘’’**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral), BBa_E1010’’’ (Endy Lab, 2004): BBa_E1010 was successfully used to produce mRFP in E.coli strain MG1655 in LB or M9 minimal media under the control of BBa_K907005.
2. Experience – ‘’’green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP’’’ (Endy Lab, 2004): BBa_E0040 was successfully used to produce GFP in E.coli strain MG1655 in LB or M9 minimal media under the control of BBa_K907004.