Team:SDU-Denmark/geneseq

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Revision as of 14:52, 26 September 2012 by Wronazky (Talk | contribs)

iGEM TEAM ::: SDU-DENMARK courtesy of NIAID



Standard restriction sites:


EcoRI:          5' GAATTC
                      3' CTTAAG

XbaI:            5' T*CTAGA
                      3' AGATC*T

SpeI:             5' A*CTAGT
                      3' TGATC*A

PstI:              5' CTGCAG
                      3' GACGTC

Our plant genes:

First primer Extended Start codon Stop codon Restriction site Mutagenesis primer 1-FFT: AGGAGGTGTCACCATGCAAACCCCTGAACCCTTTACAGACCTTGAACATGAACCCCACACACCCCTACTGGACCACCACCACAACCCACCACCACAA
ACCACCACAAAACCTTTGTTCACCAGGGTTGTGTCCGGTGTCACCTTTGTTTTATTCTTCTTTGGTTTCGCTATCGTATTCATTGTTCTCAACCAAC
AGAACTCTTCTGTTCGTATCGTCACCAATTCGGAGAAATCTTTTATAAGGTATTCGCAGACCGATCGCTTGTCGTGGGAACGGACCGCTTTTCATTT
TCAGCCTGCCAAGAATTTTATTTACGATCCAGATGGTCAGTTGTTTCACATGGGCTGGTACCATATGTTCTATCAATACAACCCATACGCACCGGTT
TGGGGCAATATGTCATGGGGTCACTCAGTGTCCAAAGACATGATCAACTGGTACGAGCTGCCAGTCGCTATGGTCCCGACCGAATGGTATGATATCG
AGGGCGTCTTATCCGGGTCTACCACGGTCCTTCCAAACGGTCAGATCTTTGCATTGTATACTGGGAACGCTAATGATTTTTCCCAATTACAATGCAA
AGCTGTACCCGTAAACTTATCTGACCCGCTTCTTATTGAGTGGGTCAAGTATGAGGATAACCCAATCCTGTACACTCCACCAGGGATTGGGTTAAAG
GACTATCGGGACCCGTCAACAGTCTGGACAGGTCCCGATGGAAAGCATAGGATGATCATGGGAACTAAACGTGGCAATACAGGCATGGTACTTGTTT
ACTATACCACTGATTACACGAACTACGAGTTGTTGGATGAGCCGTTGCACTCTGTTCCCAACACCGATATGTGGGAATGCGTCGACTTTTACCCGGT
TTCGTTAACCAATGATAGTGCACTTGATATGGCGGCCTATGGGTCGGGTATCAAACACGTTATTAAAGAAAGTTGGGAGGGACATGGAATGGATTGG
TATTCAATCGGGACATATGACGCGATAAATGATAAATGGACTCCCGATAACCCGGAACTAGATGTCGGTATCGGGTTACGGTGCGATTACGGGAGGT
TTTTTGCATCAAAGAGTCTTTATGACCCATTGAAGAAAAGGAGGATCACTTGGGGTTATGTTGGAGAATCAGATAGTGCTGATCAGGACCTCTCAAG
AGGATGGGCTACTGTTTATAATGTTGGAAGAACAATTGTACTAGATAGAAAGACCGGGACCCATTTACTTCATTGGCCCGTTGAGGAAGTCGAGAGT
TTGAGATACAACGGTCAGGAGTTTAAAGAGATCAAGCTAGAGCCCGGTTCAATCATTCCACTCGACATAGGCACGGCTACACAGTTGGACATAGTTG
CAACATTTGAGGTGGATCAAGCAGCGTTGAACGCGACAAGTGAAACCGATGATATTTATGGTTGCACCACTAGCTTAGGTGCAGCCCAAAGGGGAAG
TTTGGGACCATTTGGTCTTGCGGTTCTAGCCGATGGAACCCTTTCTGAGTTAACTCCGGTTTATTTCTATATAGCTAAAAAGGCAGATGGAGGTGTG
TCGACACATTTTTGTACCGATAAGCTAAGGTCATCACTAGATTATGATGGGGAGAGAGTGGTGTATGGGGGCACTGTTCCTGTGTTAGATGATGAAG
AACTCACAATGAGGCTATTGGTGGATCATTCGATAGTGGAGGGGTTTGCGCAAGGAGGAAGGACGGTTATAACATCAAGGGCGTATCCAACAAAAGC
GATATACGAACAAGCGAAGCTGTTCTTGTTCAACAACGCCACAGGTACGAGTGTGAAAGCATCTCTCAAGATTTGGCAAATGGCTTCTGCACCAATT
CATCAATACCCTTTTTAATTACCGGCTATCGCTATCCTTTTTGTTATTG

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