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Team:SDU-Denmark/labwork/Notebook/week8
From 2012.igem.org
Laboratory Notebook
20-08-2012 to 26-08-2012
We made a gel of the SST 1 from earlier and cut out the slice. We did miniprep on the liquid cultures from the day before (SST 2 and FFT 9) and found out that there was no product of use from the FFT vial (bacteria must have grown badly) but excellent product from SST 2 (105,3 ηg/μL first time and 82,7 ηg/μL the second time) which was marked N1 and N2. N1 was the first run of miniprep product and the N2 was the second. We made a gel where we ran the N1 SST on and cut out the smallest slice of the 2 fragments (approx. 1900 bp). The slice was extracted for DNA. We did miniprep on the liquid cultures from the day before (again) (on FFT 9) and found out that there was no product of use from the FFT vial (bacteria must have grown badly) only 4.0ηg/μL and 2.9ηg/μL in nanodrop. Therefore did we make new liquid cultures (FFT9). We ran nanodrop on the SST PCR from yesterday and got about 412-420 ηg/μL from the nanodrop. The liquid cultures form the 24th was taken out of the incubator, (after approximately 47 hours) and put in the refrigerator.
