Notebook

Our team pushed forward an experiment while making trial and error every day.

Creating parts of Tar methylation region

Date:8/9

Colony PCR

→Reflection:We Took E.coli too many.You should take less E.coli.

→Reflection:The number of cycles was less.So,We increased The number of cycles in 8/11.(50 cycles)

Date:8/11

The purified DNA

→Reflection:Band was less.

PCR Product

Date:8/13 Confirmed of electrophoresis by PCR product and Ligation of the TA vector

Date:8/14 Transformation

Date:8/25 Miniprep

Date:9/5 Miniprep,Nanodrop

Date:9/6 Miniprep,PCR,Electrophoresis

Date:9/10 Colony PCR,c,Ligation,Miniprep

Date:9/11 Primer design

Date:9/12 Miniprep,Ligation

Date:9/13 Electrophoresis,Refinement of DNA

Date:9/14 Colony PCR

Date:9/15 Ligation

Date:9/16 PCR,Ligation

Date:9/17 PCR,Ligation

Date:9/19 Ligation

Date:9/20 Enzyme inactivation,Ligation

Date:9/22 Transformation,Colony PCR,Electrophoresis,Miniprep

Date:9/23 Colony PCR,Electrophoresis,Streak

Date:9/25 Colony PCR

Creating parts of azurin

Date:8/16

Colony PCR

Ligation

Date:8/18 Colony PCR

Date:8/20 DNA extraction and purification of P.aeruginosa

Date:9/3 Ligation,Transformation,PCR,Nanodrop