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Team:UT-Tokyo/Notebook
From 2012.igem.org
Notebook
このページの概要を、簡単に記述。
May
1st week: 5/27-6/2
May 29th
- Making LB medium
May 30th
- Making LB Agar
- Making LB Amp Plates
May 31st
- Preculture
June
2nd week: 6/3-6/9
June 5th
- Plating JM109
June 6th
- preculture
June 7th
- Measuring OD by spectroscope
- Meking electrocompetent cells
- Electroporating of a test plasmid
June 8th
- Caluculatiing transformation efficiency
3rd week: 6/10-6/16
4th week: 6/17-6/23
5th week: 6/24-6/30
June 26th
- Making LB tetracycline plate
June 27th
June 28th
- Making LB kanamycin plate and chloramphenicol plate
- Transformation with a test plasmid
June 29th
- Transformation with #1(constitutive promoter) and #11(RBS)
2012/07
6th week: 7/1-7/7
July 3rd
- Making K solution
- Colony PCR DNA fragment assumed as fhlA (#6) for cloning
July 4th
- Miniprep #1, #11
July 5th
- Making 1% agarose solution
- Electrophoresis (#6)
- Digest (#1_EP, #11-_EP)
July 6th
- Colony PCR (#6)
7th week: 7/8-7/14
July 9th
- Electrophoresis (#6)
July 10th
- Transformation
- #3(RBS medium), #5(d.term), #20(pLac)
July 11th
- Preculture
- #3, #5, #20
- Gel Extraction(#6)
July 12th
- The continuation of Gel Extraction(#6)
- Miniprep
- #3, #5, #20
July 13th
- Nanodrop (#6).
- Digestion (#3:E-P, #5:E-P, #20:E-P)
- Electrophoresis (#3, #5, #20)
July 14th
- Miniprep (#1,3,5,11,20)
- Gel extraction (#6)
8th week: 7/15-7/21
July 17th
- Transformation (#1A2,#3,#5,#20)
- PCR (parts of 1. and #3,#20(Colony PCR))
- Incubate #5.
July 18th
- Miniprep (#1A2,#3,#5,#20)
- PCR using u.p (#1A2,#3,#5,#20)
- Colony PCR (#3,#20,H₂O)
- Electrophoresis (#1A2,#3,#5,#20, #3,#20, H₂O)
- PCR (2011’s #1,#36 and 2012’s #3,#5,#20, H₂O)
July 20th
- Miniprep.
- Electrophoresis (7/19’s PCR product(#1,#3,#5,#20,#36) )
- Colony PCR (#3,#5,#20)
July 21st
- Electrophoresis (7/20’s PCR product (#3,#5,#20))
9th week: 7/22-7/28
July 24th
- Making agar medium plate contain ampicillin
- PCR (#6, #8)
- Electrophoresis (#6, #8)
- Transformation (#1)
- Culture (#5, #20)
July 25th
- Miniprep (#5, #20)
- Purify (#6, #8)
- Digestion (#3:S-P,#5:E-X,#11:E-S,#20:S-P)
- Electrophoresis (#5, #11)
- Ligation (#5,#11) (?)
July 26th
- Culture (#1)
- Digestion (#3:S-P, #5:E-X, #11:E-S, #20:S-P, fhlA:E-S, hycA:E-S)
- Electrophoresis (#3:S-P, #5:E-X, #11:E-S, #20:S-P, fhlA:E-S, hycA:E-S)
- Gel extraction (#3:S-P, #5:E-X, #11:E-S, #20:S-P, fhlA:E-S, hycA:E-S)
July 27th
- Culture, Miniprep (#1)
- Digestion (#5-A:E-X, #11-A:X-P)
- Electrophoresis (#3:S-P, #20:S-P, #11:E-S)
- Gel extraction (#3:S-P, #20:S-P, #11:E-S, E-S vector, hycAp:E-S)
July 28th
ごめんなさい、何やったのかノートからではよく読み取れませんでした；；th ====
10th week: 7/29-8/4
July 30th
- Digestion (#3:S-P, #11:X-P)
- Electrophoresis (#3, #11)
- Gel extraction (#3, #11)
- Ligation (#3, #11)
- Transformation (ligation product; #3-11)
August 1th
- Digestion (#3:S-P, #11:X-P)
- Electrophoresis (#3, #11)
- Gel extraction (#3, #11)
- Ligation (#3, #11)
- Transformation (ligation product; #3-11)
- Miniprep (#3, #5, #11)
August 2th
- Colony PCR (#3-11)
- Culture (#3-11)
- Colony pick up, culture (red colony of #11)
- Digestion (#3:S-P, #5:E-X)
August 3th
- Gel extraction (#3:S-P, #5:E-X)
- Making solution of Kanamycin (100mg/mL)
- Culture, miniprep (#11)
- Culture (#11)
11th week: 7/29-8/4
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