Team:Korea U Seoul/Notebook/Aug

     raxR/H is target for raxSTAB.

     Sulfur plays an important role in activity of AX21; it won’t work without sulfur

     overexpression of raxST(sulfur attachment to AX21), RaxR/Q(transformation of PAPS into ATP) is possible in E.coli

      test any promoter which binds to raxR/H

      is it possible for Xanthomonas to produce AX21 without plant receptor?

      (Professor’s comment) we can run a test using E/coli which has plant receptor

      Method for detecting AX21 production?

      (Professor’s comment) use mass spectrometry or put florescence after ax21 gene then cut with restriction enzyme

     Find A

     find system which utilizes logic gate in natural world

     (1) research rax operon

      How to detect Ax21 : HyunKee Kim

      Research raxR/H paper : Byeongnam Min

      Research Xanthomonas : Jeongmin

      Activity of the substance only with raxR/H: Haerim Song

     (2) team

      Logic gate: Jihoon Jung, Kyeongwoo Jang

      Science camp presentation: Haerim Song

      Wiki update: Byeongnam Min, Kyeongwoo Jang, Sanghoon Han

      Notebook translation: HyunKee Kim

      Study Modeling: Sanghoon Han, Byeongnam Min

      Design team: Jeongmin Lee, Kyeongjin Kim, Jihyong Nam, Haerim Song

      raxR, raxH can be detected using His tag; however, experiments using cloning technique don’t use this.

      No indication of functional opetation only with raxR, raxH

      raxH without transmembrane segment was autophosphorylation, but no raxR transphosphrylation.

      raxH H222 and RaxR D51 is responsible for phosphorylation.

      acquisition of PCEL, raxRH promoter, mRFP using PCR

      cloning is expected to be held this week

      raxR/H is constitutive, so use other promoter

     logic gate: find A: Sanghoon Han, Jihoon Jung, Kyeongwoo Jang

     Ax21 production: Wonwook Lee, JungMin, Kyeongjin, Haerim Song, Byeongnam Min, Hyunkee Kim

     Brochure: Jeongmin Lee, Kyeongjin Kim, Jihyong Nam, Haerim Song

     Science camp presentation: Haerim Song

     Wiki update: Nam, Byeongnam Min, Kyeongwoo Jang, Sanghoon Han

     Notebook translation: HyunKee Kim

     Study Modeling: Sanghoon Han, Byeongnam Min

     Design team: Jeongmin Lee, Kyeongjin Kim, Jihyong Nam, Haerim Song

     CCP: Sanghoon Han

     KAIST Contact; Byeongnam Min

     Team attire: Jihoon Jung

     upload at least one copy of design to the homepage (mandatory)

      researched logic gate used by organism to detect certain chemical

      British Columbia University already did similar project; it was about concentration dependent color changing bacteria.

      Logic gate using single step is more efficient than using several input and output steps

      DSF(diffusible signaling factor)

      had presentation about iGEM during ‘Lifescience camp’

      in order to prevent bacterial leaf blight, chemical-pesticide was given. However, it was proven to be ineffective.

      Bacterial leaf blight resistant plant was developed but, later sub-population eliminated effect of ‘gene R.’

      LG lifescience, Celltrion, Rural development administration, cosmogenetech: yet positive reply, he will continue to contact.

      Small Protein-Mediated Quorum Sensing in a Gram- Negative Bacterium (PMID : 22174954) To elucidate this function, we previously isolated and characterized eight genes required for Ax21 activity (rax genes). raxA, raxB and raxC encode components of a predicted type I secretion system (TOSS). Ax21requires this RaxABC TOSS for activity and secretion [3,4]. The RaxB protein carries two highly conserved N terminal proteolytic subdomains characteristic of transporters in Gram-positive bacteria that cleave N-terminal peptides prior to substrate secretion [4]. These data, together with the presence of a predicted N-terminal signal sequence in Ax21, suggest that Ax21 is cleaved by the RaxB transporter prior to secretion. raxST, raxP and raxQ encode enzymes involved in sulfation

      This indicates AX21 is synthesized without a plant

     Benchmark : http://www.linocoms.co.kr/index.htm

     need to use ‘Flash’ program

      Regional Jamboree registration : 275 USD

      Airfare : 40~50 KRW

      Hotel reservation : iGEM will propose several hotels in a discount charge

     Sanghoon Han : CCP report, video making, researching logic gate background & design, modeling, Note-book, protocol production

     Jihoon Jung : CCP report, video making, uniform order(later)

     Kyeongwoo Jang: CCP report , video making, logic gate background & design

     Jeongmin Lee: Xanthomonas Abstract & background

     Hyunkee Kim : Xanthomonas Abstract & background, Note-book, Safety question

     Haerim Song: mainframe design, poster design(later)

     Byeongnam Min: modeling

     Jihyong Nam: protocol

     Kyeongjin Kim: get sponsor

     Wonwook Lee : get sponsor