Team:Nanjing China Bio/Safety2

From 2012.igem.org

Revision as of 07:53, 26 September 2012 by Leciel7 (Talk | contribs)

SafetyHome > results

To confirm the distribution of VNP20009 in mouse tissues and to verify the tumor-specific gene induction, we detected GFP expression in tumor, liver, and spleen by fluorescence microscopy. We found the VNPpLacZ-GFP (with constitutive promoter) could induce expression not only in necrotic tumor but also in spleen and liver tissue. However, VNPpNirBeGFP (with tumor specific promoter) could only be induced in hypoxic and necrotic tumor tissue.