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Team:Macquarie Australia/Protocols/RD
From 2012.igem.org
Revision as of 01:19, 26 September 2012 by
Ryankenny
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Restriction Digest
We prepared Master Mixes containing to following volumes,
EcoR1 + Spe1 Master Mix
Substrate
Volume (µL)
10x Buffer
20
EcoR1
2.5
Spe1
2.5
Water
75
Total
100
EcoR1 + Spe1 Master Mix
Substrate
Volume (µL)
10x Buffer
20
EcoR1
2.5
Spe1
2.5
Water
75
Total
100
EcoR1 + Spe1 Master Mix
Substrate
Volume (µL)
10x Buffer
20
EcoR1
2.5
Spe1
2.5
Water
75
Total
100
EcoR1 + Spe1 Master Mix
Substrate
Volume (µL)
10x Buffer
20
EcoR1
2.5
Spe1
2.5
Water
75
Total
100
"
