Team:Potsdam Bioware/Project/Part Antibody

Antibody Module

Introduction

Aim of the Antibody Module

The idea of the antibody module is to create antibody constructs which will specifically be mutated and optimized by the AID (activation induces deaminase). Our two antibody constructs allow an easy handling and a straightforwardly integration into CHO cells. The use of Flp-In system realizes time saving incorporation into the CHO genome. A switch from surface presentation to secretion of the antibodies can be enabled by TEV protease and cre recombinase and permits a comfortable harvest of selected antibodies.