Team:SDU-Denmark/labwork/Notebook/week4

Laboratory Notebook

Here you find the log book for the procedures carried out in the laboratory, starting from week 27.

23-07-2012:

SST send for sequencing

SST from the gel extraction the 21-07-2012 was diluted to a concentration of 100ng/µL and separated to 4 samples of 15µL each and send of for sequencing.
We made liquid cultures from the SST plates from 19-07-2012 and incubated them O.N.

24-07-2012:

Cryo backup, miniprep and preparation to sequencing

We made some Cryo backup’s from the 18 liquid cultures, that was incubated O.N. and took some sample out from each to do a miniprep on.
We ran a gel on the samples and found that two samples had bands corresponding to our cut gene and vector. These will be shipped of to be sequneced tomorrow.

25-07-2012:

SST send for sequencing

The two sample from the day before were prepared for sequencing by diluting them to 70-78 ng/μl and sent them for sequencing. Afterward we looked into the expected outcomes of sequencing and had a look at the following mutagenesis post-sequencing.