BBa_K875006

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Description

This construct is designed for the expression of secreted, already described engineered antinorovirus (NoV) monoclonal antibody (mAb 54.6).
The antibody is expressed in a single chain fragment variable (scFv) format containing light (VL) and heavy (VH) variable domains separated by a flexible peptide linker. It has already been reported that the scFv 54.6 binds a native recombinant NoV particles (VLPs) and inhibits VLP interaction with cells. PelB is a leader sequence often used to produce secreted proteins.

The construct consistes of T5 Lac Operator (Bba_K875002), ribosomal binding site, PelB-scFv 54.6 antinorovirus, HIstidine tag (6HIS), Terminator (B0015).

The expression of the protein PelB-scFv 54.6-HIS is regulated by T5 Lac Operator (Bba_K875002). When the promote T5 Lac O is induced with IPTG (1mM), scFv 54.6 is expected to be secreted. PelB drives the protein into the periplasmic space where is clived off. The resulting protein then can be released into extracellular space by passing through porins.
Molecular Weight: 29,25 kDa.