Team:Exeter/lab book/3gip/wk6

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ExiGEM2012 Lab Book 3GP wk6


Protocols \| Single Gene Plasmids and Enzyme Characterisation \| Showcasing Polysaccharide Production \| The 3-Gene Inducible Plasmid Operon Construction \| Glycobase
9th - 13th July - 16th - 20th July - 23rd - 27th July - 30th July - 3rd August - 6th - 10th August - 13th - 17th August - 20th - 24th August - 27th - 31st August - 3rd - 7th September - 10th - 14th September - 17th - 21st September
	The 3-Gene Inducible Plasmid: 13th - 17th August 2012 Monday 13th August See Operon Construction: 13th - 17th August 2012 https://2012.igem.org/Team:Exeter/lab_book/gibs/wk6 Tuesday 14th August See Operon Construction: 13th - 17th August 2012 https://2012.igem.org/Team:Exeter/lab_book/gibs/wk6 Wednesday 15th August Morning •Mini-Prepping BBa_J13002 + WbnK_BBa_B0014 in pSB1T3 WfcA + BBa_B0014 in pSB1T3 WbnJ + BBa_B0014 in pSB1T3 BBa_B0034_WbbC + BBa_B0014 in pSB1T3 Using the changed protocol. The tubes were centrifuged for 10 minutes to start to ensure a goo pellet formed. After the neutralisation solution had been added, the eppindorfs were centrifuged for 10 minutes rather than 5 in order to get a better pellet formation. 40ul of water was added instead of 40ul in order to make sure the concentration of DNA increased. All were nanodropped and only WfcA + BBa_B0014 and WbnJ + BBa_B0014 gave good concentrations. Wednesday 15th August –Friday 17th August Preparation of presentation so no lab work.