"
Page
Discussion
View source
History
teams
Log in
Team:XMU-China/protocolsindex
From 2012.igem.org
Revision as of 15:46, 25 September 2012 by
FBS
(
Talk
|
contribs
)
(
diff
)
← Older revision
|
Latest revision
(
diff
) |
Newer revision →
(
diff
)
safetyindex
Contents
[hide]
[show]
1 General protocols
1.1 Stock solution
1.2 Preparation of Competent BL21
1.3 Transformation
1.4 Plasmid Purification
1.5 Reaction system of restriction endonuclease
1.6 Standard BioBrick Assembly
1.7 Suffix Insertion
1.8 Ligation
1.9 Restriction analysis
1.10 Gel Extraction
2 Characterization
2.1 Fluorescence Measurements
2.2 Protein electrophoresis
2.2.1 Preparation of Samples
2.2.2 Manufacture Albumen Gel
2.2.3 Electrophoresis
2.2.4 Dyeing (Colloidal Coomassie Brilliant Blue)
2.2.5 Scanning
3 Immobilization
3.1 Prepare Sodiumcellu-losesulfate (NaCS)
3.2 Prepare microcapsules
3.3 Immobilize cells into calcium alginate beads
3.4 Immobilize cells into intra-hollow Ca-alginate capsules
3.5 Immobilize cells into NaCS-PDMDAAC microcapsules