Team:MIT/Results
From 2012.igem.org
RNA Strand Displacement In Vitro
Nucleic Acid Delivery
In Vivo RNA Strand Displacement
Strategy 1: Take DNA sequences that worked in In Vitro Strand Displacement, convert to RNA, transfect inside HEK293 cells
Strategy 2: Tag RNA strand with an Alexa Fluorophore to act as a transfection marker
Strategy 3: Create DNA plasmids driving transcription of RNA inputs, while transfecting RNA Reporter
Strategy 4: Nucleofect RNA reporter, RNA inputs
[[Strategy 5]]: Redesign RNA Reporter
Strategy 1: Take DNA sequences that worked in In Vitro Strand Displacement, convert to RNA, transfect inside HEK293 cells
Strategy 2: Tag RNA strand with an Alexa Fluorophore to act as a transfection marker
Strategy 3: Create DNA plasmids driving transcription of RNA inputs, while transfecting RNA Reporter
Strategy 4: Nucleofect RNA reporter, RNA inputs
[[Strategy 5]]: Redesign RNA Reporter