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From 2012.igem.org

week 9: 25 june - 1 july

Office work

Lab work

Biobrick BBa_B0015 (a terminator) was cloned.

TuYV

Wednesday:

• Last week's PCR products(1,1H,2,2H,3,3H) were digested, ligated into the linearized pSB1A3 backbone and transformed into our electrocompetent DH5 Alfa cells.

Then after transformation, we checked the colonies on the plate, but only few colonies on positive control and sample 1(TuYV coat protein), 1H(TuYV coat protein his-tag) and 2 plate (TuYV coat protein + 1/2 readthrough).

Friday:

• Colonies from 1, 1H and 2 plate were picked and checked by colony PCR. But no bands were showed later on the agrose gel check.

Written by: Han Yue

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