General PCR Protocol

Set up PCR reaction tube

1. 1. 1uL Template
   2. 1uL 25mM dNTP's
   3. 10uL Buffer
   4. 0.5uL Forward Primer
   5. 0.5uL Reverse Primer
   6. 0.5uL TAQ polymerase
   7. 36.5uL diH2O

Amplification reaction in thermocycler

1. 1. 1 cycle 94 degrees (3 minutes)
   2. 40 cycles 94 degrees (1 minute), 50 degrees (1 minute), 72 degrees (1 minute)

Source

Thanks to UW iGEM '11 for the above instructions. The original source can be found here: University Washington gel electrophoresis protocol