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From 2012.igem.org

Nine Gibson Assembly Reactions

	3A	3K	3C	4A	4K	4C	5A	5K	5C
1. Addition of 20ng gene fragments in appropriate tube	2 μL each of Deino_A, Deino_B, Deino_C, Deino_D, Deino_E	2 μL each of Deino_A, Deino_B, Deino_C, Deino_D, Deino_E	2 μL each of Deino_A, Deino_B, Deino_C, Deino_D, Deino_E	2 μL each of AgroT7_A, Agro_B, Agro_C, Agro_D, Agro_E	2 μL each of AgroT7_A, Agro_B, Agro_C, Agro_D, Agro_E	2 μL each of AgroT7_A, Agro_B, Agro_C, Agro_D, Agro_E	2 μL each of Agro_A, Agro_B, Agro_C, Agro_D, Agro_E	2 μL each of Agro_A, Agro_B, Agro_C, Agro_D, Agro_E	2 μL each of Agro_A, Agro_B, Agro_C, Agro_D, Agro_E
2. Addition of 0.05pmol of vector	2.8 μL PSB-1A3	2.9 μL PSB-1K3	2.7 μL PSB-1C3	2.8 μL PSB-1A3	2.9 μL PSB-1K3	2.7 μL PSB-1C3	2.8 μL PSB-1A3	2.9 μL PSB-1K3	2.7 μL PSB-1C3
3. Addition of Master Mix (μL)	12.8	12.9	12.7	12.8	12.9	12.7	12.8	12.9	12.7