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From 2012.igem.org

SUMMARY OF IT ALL HERE; similar to front page

Contents 1 Origins of the Idea 2 Preliminary Lab 3 Lab Work 4 Future Experiments 5 Future Applications

Origins of the Idea

. Needed a way to quantitatively measure the output of NO with non-specific promoters, decided to look at a gene regulation system

. Looked at a way for other molecules resulting in transcription to knock one another out

Preliminary Lab

. Designed DNA constructs for the subtractive system and had them synthesised

Lab Work

. Made into biobricks (link to registry page)

Future Experiments

. Ligating with different promoters and reporters to test

. Ultimately ligate with NO-sensing promoters and effecter enzymes to control NO levels/reporters to detect NO levels quantitatively

Future Applications

. Diabetes

. Cancer

. Environment/Pollution