Team:WashU/Protocols/PCR

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General PCR Protocol

  1. Set up PCR reaction tube
    1. 1uL Template
    2. 1uL 25mM dNTP's
    3. 10uL Phusion HF Buffer
    4. 0.5uL Forward Primer (Tm XX oC)*
    5. 0.5uL Reverse Primer (Tm YY oC)*
    6. 0.5uL TAQ polymerase
    7. 36.5uL diH2O
  1. Amplification reaction in thermocycler depends
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