Team:Peking/DataPage

Data for favorite parts

Optimized Luminesensor(BBa_K819005) a fusion protein consisting of an E.coli endogenous SOS system repressor LexA, carrying mutations at positions 40-42, and fungus N. crass photosensor protein VVD, carrying mutations N56K, C71V and I135M. This part serves as an ultra-sensitive photoreceptor which can sense light as weak as moonlight and will induce light-dependent repression of genes with their promoters containing mutated 408 forms of the SOS box.

Testing device for Luminesensor(BBa_K819006) and measurement device for Luminesensor(BBa_K819007) a fast degrading GFP ligated to the downstream of SulA and RecA promoters, both in 408 form (only recognizable by our LexA-VVD fusing protein, not by E.coli endogenous LexA). The SulA promoter, which belongs to the SOS regulon family, promotes the expression of SulA protein gene which expresses SulA protein, a cell division inhibitor. The RecA promoter promotes the expression of RecA protein, which cuts the dimerized LexA protein in response to double strand break. When E.coli cells co-transformed with our luminesensor plasmid and light-responsive reporter plasmids are illuminated by blue light, the LexA408-VVD fusion protein expressed within the cell will dimerize in response to the light. The dimerized LexA408 domains will bind the SOS box (in 408 form) of both the SulA408 promoter and RecA408 promoter to inhibit the transcription of the downstream gene so that no GFP will be expressed. If the surrounding environment is dark, the luminesensor will not dimerize and no supression of the promoters will occur, leading to the expression of GFP.