Team:Cambridge/Protocols/M9Medium

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M9 Minimal Medium

M9 salts x5

  • 64 g Na2HPO4.7H2O
  • 15 g KH2PO4
  • 2.5 g NaCl
  • 5 g NH4Cl

dissolved in deionized water to a final volume of 1 L

Autoclave before use.

Final medium

  • 1x M9 salts
  • 2mM MgSO4
  • 0.1mM CaCl2
  • 0.4% carbon source (e.g. glycerol, glucose, etc.)
  • In sterile H2O
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