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Team:SDU-Denmark/Project/Future
From 2012.igem.org
Future Prospects
Our idea to battle obesity gives rise to a lot of different aspects of the future. In this section we will try to describe some of the ideas we had for future applications. When devising these future ideas, we have always had in mind, the safety considerations needed for GMO. Therefore this section will begin with our killswitch.
Killswitch
During the construction of killswitch, we stumbled upon a very concerning fact. A normal killswitch inducible by a promoter can be rendered useless in the case of frameshift mutations (and any other mutation, that affects the gene-product). Therefore we chose to construct killswitches with a less likely chance of being frameshifted out of order. We have come up with two solutions, where one of them, is new to us. We have named the two concepts: The risky concept and the safe concept. This is simply because our knowledge about the risky concept is limited and only theoretically plausible. The safe concept is seen before, at least bits of it. Both of the constructs are supposed to be integrated into the genome of the bacteria in order to avoid selection pressure. You can read about the idea of our killswitches below. The risky concept
Fmet1b= frameshifted met codon (1 basepair) F1bp stop= Frameshifted 1 basepair stopcodon Fmet2b= frameshifted met codon (2 basepair) F2bp stop= Frameshifted 2 basepair stopcodon
In this risky concept we have chosen to introduce frameshifted versions of our death gene, ccdB, thereby allowing the genes to be activated upon frameshift, keeping the killswitch functional. This is, as mentioned earlier, only theoretically possible and would need testing before introducing it into our product. The promoter is activated by the presence of the synthetic sugar, L-rhamnose, which is indigestible to humans. This allows the consumer to drink a glass of water with dissolved L-rhamnose in order to kill the GMO bacteria introduced by the yoghurt.
The safe concept
