"
Team:Macquarie Australia/trial
From 2012.igem.org
To browse through our notebook simply click on the image with the date. It will expand with what we did that week. To continue on simply click on the next one.
With the break between semesters over, the Macquarie iGEM returned to classes. For us this was the first day of iGEM 2012. We had our first meeting and discussed our project. Dr. Louise Brown and Associate Professor Rob Willows were introduced the team and we were began to determine who would take on certain roles within the team.
We eagerly began our project, deciding to use the novel approach of Gibson Assemble to produce our optimised genes. We had decided to develop a gene-switch controlled by light. To do this a couple of genes would be needed:
- a bacteriophytochrome
- Heme oxygenase
The bacteriophytochromes from Deinococcus radiodurans and Agrobacterium tumefaciens were chosen. Over the next week Matt Stclair started to develop the G-blocks by:
- Acquiring the DNA sequence
- Translating into the protein sequence
- Using the DNA sequence, optimise codon usage for E. coli
- Translating into protein sequence and ensuring no changes in amino acids relative to the original sequence
Our lab work began today with the preparation of liquid media, plates and buffers. The protocols we followed are located here.
We prepared the following:
- Liquid LB Media
- SOC Solution
- SOB Solution
- LB Agar Plates
- Ampicillin LB Agar Plates: 31 plates
- Chloramphenicol LB Agar Plates: 33 Plates
- Kanamyacin LB Agar Plates: 32 Plates
- TB buffer.
- TAE buffer.
- EDTA buffer.
While the lab group produced the necessary materials, another team began developing the G Blocks. The G Block team consisted of Ellaina, Matt Smith, Matt Stclair, Harry, Gazelle, Silas, Kim, and Miguel. Matt Stclair spearheaded the effort and proofread all the sequences we had developed to ensure that we had not changed the protein sequence or introduced restriction sites.
Ryan volunteered to be the wiki chief with Erin helping out throughout the project. Ellaina ensured the key safety questions were answered, and relayed these to the rest of the team.
In a Macquarie iGEM first, the team took a big interest in the idea of human outreach and we had a brainstorming session. Ellaina proposed that we visit a high school while Elle suggested that the Universities open day would be a great opportunity to communicate with the wider community. Ellaina took control of developing our high school visit and Elle took control of Open Day.
In anticipation of our G Blocks arriving next week, competent cells were prepared. The protocol followed can be seen here.
Human Practice planning went into overdrive this week. To engage the wider community, fluorescent and bioluminescent parts from the registry were selected and transformed in E. coli.
This is a trial. I do not know if this is going to work or not!
This is a trial. I do not know if this is going to work or not!
This is a trial. I do not know if this is going to work or not!
This is a trial. I do not know if this is going to work or not!
This is a trial. I do not know if this is going to work or not!
This is a trial. I do not know if this is going to work or not!
